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Image Search Results
Journal: Cancer Research
Article Title: The BH3 Mimetic ABT-737 Induces Cancer Cell Senescence
doi: 10.1158/0008-5472.can-10-1977
Figure Lengend Snippet: Figure 1. Gene transcription changes after ABT-737 treatment. A, microarray analysis of gene expression induced by ABT-737. PV-10 cells were treated in triplicate with DMSO or 10 mmol/L ABT-737 for 24 hours and gene microarray changes documented. B, gene changes associated with senescence. C, qT-PCR analysis of IL-6 and IL-8 transcripts. PV-10 and 22Rv1 cells were treated in triplicate with DMSO, ABT-737 (10 mmol/L) or enantiomer (En., 10 mmol/ L) for 24 hours (mean SD, n ¼ 3). D, the secretion of IL-6 and IL-8 in PV-10 cells treated with DMSO, ABT-737 or enantiomer for 24 hours was determined by ELISA (n ¼ 6, mean SD).
Article Snippet: Conditioned medium was collected following treatment and used at a 2:1 dilution in the human interleukin-6 (IL-6) or
Techniques: Microarray, Gene Expression, Enzyme-linked Immunosorbent Assay
Journal: Journal of Inflammation Research
Article Title: Protective Effects of Qingre Sanjie Jiaonang on Pulmonary Fibrosis: A Pilot Study
doi: 10.2147/JIR.S479432
Figure Lengend Snippet: QRSJ alleviated the release of inflammatory factors during the progression of PF. ( A ) Immunofluorescence staining was performed, with imaging for NE (red), and staining with DAPI for nucleus (blue). Scale bar, 50 μm or 20 μm (Zoom). ( B ) Quantitative analysis of the mean fluorescence intensity of NE (n = 3). ( C ) The effect of QRSJ on the levels of NE in serum in different groups using ELISA (n = 5). ( D-F ) The effect of QRSJ on the levels of CXCL15, IL-1β and TNF-α in BALF in different groups using ELISA (n = 3). ( G ) The effect of QRSJ on the levels of IL-6 in serum in different groups using ELISA (n = 5). ( H ) The effect of QRSJ on the levels of CXCL15 in serum in different groups using ELISA (n = 4). ( I ) The effect of QRSJ on the levels of TNF-α in serum in different groups using ELISA (n = 5). The data is presented as mean ± SD, * p < 0.05, ** p < 0.01 and *** p < 0.001 by one-way ANOVA. ns represents no significant difference.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) Kits for tumor necrosis factor (TNF)-α (Cat # E-EL-M3063), IL-6 (Cat # E-EL-M0044),
Techniques: Immunofluorescence, Staining, Imaging, Fluorescence, Enzyme-linked Immunosorbent Assay